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    Evident Corporation algorithm olympus cellsens dimensions olympus n a software
    Algorithm Olympus Cellsens Dimensions Olympus N A Software, supplied by Evident Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/algorithm+olympus+cellsens+dimensions+olympus+n+a+software/cellSens+Imaging+Software/10__7554_slash_elife__78984-361-100-101
    Average 99 stars, based on 1 article reviews
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    Recombinant:

    Article Title: Molecular dissection of condensin II-mediated chromosome assembly using in vitro assays
    Article Snippet: Reagent type (species) or resource Designation Source or reference Identifiers Additional information Cell line (Spodoptera frugiperda) Sf9 insect cells ThermoFisher 11496015 Cell line (Spodoptera frugiperda) High five insect cells ThermoFisher B85502 Strain, strain background (Escherichia coli) Max efficiency DH10Bac competent cells ThermoFisher 10361012 Strain, strain background (Escherichia coli) BL21(DE3) competent cells with lab cultured cells N/A Electrocompetent cells Biological sample (Xenopus laevis) Xenopus laevis egg Hamamatsu SeibutsuKyozai RRID: NXR 0.031 Female, adult frogs Biological sample (Xenopus laevis) Xenopus laevis sperm nuclei Hamamatsu SeibutsuKyozai RRID: NXR 0.031 Male, adult frogs Biological sample (Mus musculus) Mus musculus sperm nuclei Shintomi et al., 2017; isolated from Mus musculus cauda epididymis (BALB/ c×C57BL/6 J)F1 N/A Male, adult mouse Antibody anti- Xenopus topoisomerase IIα (rabbit polyclonal serum) Hirano and Mitchison, 1994 in house: αC1- 6 WB (1/2000) Antibody anti- XCAP- D2 (rabbit polyclonal) Hirano et al., 1997 in house: AfR16L WB (1.0 μg/mL) Antibody anti- XCAP- G (rabbit polyclonal) Hirano et al., 1997 in house: AfR113L WB (1.0 μg/mL) Yoshida et al. eLife 2022;11:e78984. .. DOI: https://doi.org/10.7554/eLife.78984 19 of 27 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Peptide, recombinant protein EcoRI restriction enzyme TaKaRa 1040 Peptide, recombinant protein NotI restriction enzyme TaKaRa 1166 Peptide, recombinant protein SpeI restriction enzyme TaKaRa 1086 Peptide, recombinant protein T4 polynucleotide kinase TaKaRa 2021 Commercial assay or kit QuikChange II XL Site- Directed Mutagenesis Kit Agilent Technologies 200522 Commercial assay or kit DNA Ligation Kit TaKaRa 6022 Commercial assay or kit EnzChek Phosphate Assay Kit ThermoFisher E6646 Software, algorithm UNICORN 7 Cytiva N/A Software, algorithm Prism 8 GraphPad N/A Software, algorithm Excel Microsoft N/A Software, algorithm Olympus cellSens Dimensions Olympus N/A Software, algorithm Photoshop Adobe N/A Software, algorithm ImageJ Schneider et al., 2012 https://imagej.nih. gov/ij/ Software, algorithm SparkControl Tecan Life Sciences N/A Other Coomassie Brilliant Blue R- 250 staining solution Bio- Rad 1610436 For polyacrylamide gels Other Amersham Protran Premium 0.45 nitrocellulose membrane Cytiva 10600047 For Western blots Other Glutathione Sepharose 4B Cytiva 17075601 Used for purification of condensin I Other HiTrap Q HP 1 mL Cytiva 17115301 Used for purification of condensins Other StrepTactin Sepharose HP Cytiva 28- 9355- 99 Used for purification of condensin II Other DAPI Roche 10236276001 (2 μg/mL) Other ATP Sigma- Aldrich A2383 Used in ATPase assay and Npm2- treated chromatin binding assay Other Dynabeads Protein A ThermoFisher 10002D For immunodepletion with Xenopus egg extract Other Alexa Flour 488- conjugated streptavidin ThermoFisher S32354 IF (1/2000) SMC: structural maintenance of chromosomes. ..

    Mutagenesis:

    Article Title: Molecular dissection of condensin II-mediated chromosome assembly using in vitro assays
    Article Snippet: Reagent type (species) or resource Designation Source or reference Identifiers Additional information Cell line (Spodoptera frugiperda) Sf9 insect cells ThermoFisher 11496015 Cell line (Spodoptera frugiperda) High five insect cells ThermoFisher B85502 Strain, strain background (Escherichia coli) Max efficiency DH10Bac competent cells ThermoFisher 10361012 Strain, strain background (Escherichia coli) BL21(DE3) competent cells with lab cultured cells N/A Electrocompetent cells Biological sample (Xenopus laevis) Xenopus laevis egg Hamamatsu SeibutsuKyozai RRID: NXR 0.031 Female, adult frogs Biological sample (Xenopus laevis) Xenopus laevis sperm nuclei Hamamatsu SeibutsuKyozai RRID: NXR 0.031 Male, adult frogs Biological sample (Mus musculus) Mus musculus sperm nuclei Shintomi et al., 2017; isolated from Mus musculus cauda epididymis (BALB/ c×C57BL/6 J)F1 N/A Male, adult mouse Antibody anti- Xenopus topoisomerase IIα (rabbit polyclonal serum) Hirano and Mitchison, 1994 in house: αC1- 6 WB (1/2000) Antibody anti- XCAP- D2 (rabbit polyclonal) Hirano et al., 1997 in house: AfR16L WB (1.0 μg/mL) Antibody anti- XCAP- G (rabbit polyclonal) Hirano et al., 1997 in house: AfR113L WB (1.0 μg/mL) Yoshida et al. eLife 2022;11:e78984. .. DOI: https://doi.org/10.7554/eLife.78984 19 of 27 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Peptide, recombinant protein EcoRI restriction enzyme TaKaRa 1040 Peptide, recombinant protein NotI restriction enzyme TaKaRa 1166 Peptide, recombinant protein SpeI restriction enzyme TaKaRa 1086 Peptide, recombinant protein T4 polynucleotide kinase TaKaRa 2021 Commercial assay or kit QuikChange II XL Site- Directed Mutagenesis Kit Agilent Technologies 200522 Commercial assay or kit DNA Ligation Kit TaKaRa 6022 Commercial assay or kit EnzChek Phosphate Assay Kit ThermoFisher E6646 Software, algorithm UNICORN 7 Cytiva N/A Software, algorithm Prism 8 GraphPad N/A Software, algorithm Excel Microsoft N/A Software, algorithm Olympus cellSens Dimensions Olympus N/A Software, algorithm Photoshop Adobe N/A Software, algorithm ImageJ Schneider et al., 2012 https://imagej.nih. gov/ij/ Software, algorithm SparkControl Tecan Life Sciences N/A Other Coomassie Brilliant Blue R- 250 staining solution Bio- Rad 1610436 For polyacrylamide gels Other Amersham Protran Premium 0.45 nitrocellulose membrane Cytiva 10600047 For Western blots Other Glutathione Sepharose 4B Cytiva 17075601 Used for purification of condensin I Other HiTrap Q HP 1 mL Cytiva 17115301 Used for purification of condensins Other StrepTactin Sepharose HP Cytiva 28- 9355- 99 Used for purification of condensin II Other DAPI Roche 10236276001 (2 μg/mL) Other ATP Sigma- Aldrich A2383 Used in ATPase assay and Npm2- treated chromatin binding assay Other Dynabeads Protein A ThermoFisher 10002D For immunodepletion with Xenopus egg extract Other Alexa Flour 488- conjugated streptavidin ThermoFisher S32354 IF (1/2000) SMC: structural maintenance of chromosomes. ..

    DNA Ligation:

    Article Title: Molecular dissection of condensin II-mediated chromosome assembly using in vitro assays
    Article Snippet: Reagent type (species) or resource Designation Source or reference Identifiers Additional information Cell line (Spodoptera frugiperda) Sf9 insect cells ThermoFisher 11496015 Cell line (Spodoptera frugiperda) High five insect cells ThermoFisher B85502 Strain, strain background (Escherichia coli) Max efficiency DH10Bac competent cells ThermoFisher 10361012 Strain, strain background (Escherichia coli) BL21(DE3) competent cells with lab cultured cells N/A Electrocompetent cells Biological sample (Xenopus laevis) Xenopus laevis egg Hamamatsu SeibutsuKyozai RRID: NXR 0.031 Female, adult frogs Biological sample (Xenopus laevis) Xenopus laevis sperm nuclei Hamamatsu SeibutsuKyozai RRID: NXR 0.031 Male, adult frogs Biological sample (Mus musculus) Mus musculus sperm nuclei Shintomi et al., 2017; isolated from Mus musculus cauda epididymis (BALB/ c×C57BL/6 J)F1 N/A Male, adult mouse Antibody anti- Xenopus topoisomerase IIα (rabbit polyclonal serum) Hirano and Mitchison, 1994 in house: αC1- 6 WB (1/2000) Antibody anti- XCAP- D2 (rabbit polyclonal) Hirano et al., 1997 in house: AfR16L WB (1.0 μg/mL) Antibody anti- XCAP- G (rabbit polyclonal) Hirano et al., 1997 in house: AfR113L WB (1.0 μg/mL) Yoshida et al. eLife 2022;11:e78984. .. DOI: https://doi.org/10.7554/eLife.78984 19 of 27 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Peptide, recombinant protein EcoRI restriction enzyme TaKaRa 1040 Peptide, recombinant protein NotI restriction enzyme TaKaRa 1166 Peptide, recombinant protein SpeI restriction enzyme TaKaRa 1086 Peptide, recombinant protein T4 polynucleotide kinase TaKaRa 2021 Commercial assay or kit QuikChange II XL Site- Directed Mutagenesis Kit Agilent Technologies 200522 Commercial assay or kit DNA Ligation Kit TaKaRa 6022 Commercial assay or kit EnzChek Phosphate Assay Kit ThermoFisher E6646 Software, algorithm UNICORN 7 Cytiva N/A Software, algorithm Prism 8 GraphPad N/A Software, algorithm Excel Microsoft N/A Software, algorithm Olympus cellSens Dimensions Olympus N/A Software, algorithm Photoshop Adobe N/A Software, algorithm ImageJ Schneider et al., 2012 https://imagej.nih. gov/ij/ Software, algorithm SparkControl Tecan Life Sciences N/A Other Coomassie Brilliant Blue R- 250 staining solution Bio- Rad 1610436 For polyacrylamide gels Other Amersham Protran Premium 0.45 nitrocellulose membrane Cytiva 10600047 For Western blots Other Glutathione Sepharose 4B Cytiva 17075601 Used for purification of condensin I Other HiTrap Q HP 1 mL Cytiva 17115301 Used for purification of condensins Other StrepTactin Sepharose HP Cytiva 28- 9355- 99 Used for purification of condensin II Other DAPI Roche 10236276001 (2 μg/mL) Other ATP Sigma- Aldrich A2383 Used in ATPase assay and Npm2- treated chromatin binding assay Other Dynabeads Protein A ThermoFisher 10002D For immunodepletion with Xenopus egg extract Other Alexa Flour 488- conjugated streptavidin ThermoFisher S32354 IF (1/2000) SMC: structural maintenance of chromosomes. ..

    Software:

    Article Title: Molecular dissection of condensin II-mediated chromosome assembly using in vitro assays
    Article Snippet: Reagent type (species) or resource Designation Source or reference Identifiers Additional information Cell line (Spodoptera frugiperda) Sf9 insect cells ThermoFisher 11496015 Cell line (Spodoptera frugiperda) High five insect cells ThermoFisher B85502 Strain, strain background (Escherichia coli) Max efficiency DH10Bac competent cells ThermoFisher 10361012 Strain, strain background (Escherichia coli) BL21(DE3) competent cells with lab cultured cells N/A Electrocompetent cells Biological sample (Xenopus laevis) Xenopus laevis egg Hamamatsu SeibutsuKyozai RRID: NXR 0.031 Female, adult frogs Biological sample (Xenopus laevis) Xenopus laevis sperm nuclei Hamamatsu SeibutsuKyozai RRID: NXR 0.031 Male, adult frogs Biological sample (Mus musculus) Mus musculus sperm nuclei Shintomi et al., 2017; isolated from Mus musculus cauda epididymis (BALB/ c×C57BL/6 J)F1 N/A Male, adult mouse Antibody anti- Xenopus topoisomerase IIα (rabbit polyclonal serum) Hirano and Mitchison, 1994 in house: αC1- 6 WB (1/2000) Antibody anti- XCAP- D2 (rabbit polyclonal) Hirano et al., 1997 in house: AfR16L WB (1.0 μg/mL) Antibody anti- XCAP- G (rabbit polyclonal) Hirano et al., 1997 in house: AfR113L WB (1.0 μg/mL) Yoshida et al. eLife 2022;11:e78984. .. DOI: https://doi.org/10.7554/eLife.78984 19 of 27 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Peptide, recombinant protein EcoRI restriction enzyme TaKaRa 1040 Peptide, recombinant protein NotI restriction enzyme TaKaRa 1166 Peptide, recombinant protein SpeI restriction enzyme TaKaRa 1086 Peptide, recombinant protein T4 polynucleotide kinase TaKaRa 2021 Commercial assay or kit QuikChange II XL Site- Directed Mutagenesis Kit Agilent Technologies 200522 Commercial assay or kit DNA Ligation Kit TaKaRa 6022 Commercial assay or kit EnzChek Phosphate Assay Kit ThermoFisher E6646 Software, algorithm UNICORN 7 Cytiva N/A Software, algorithm Prism 8 GraphPad N/A Software, algorithm Excel Microsoft N/A Software, algorithm Olympus cellSens Dimensions Olympus N/A Software, algorithm Photoshop Adobe N/A Software, algorithm ImageJ Schneider et al., 2012 https://imagej.nih. gov/ij/ Software, algorithm SparkControl Tecan Life Sciences N/A Other Coomassie Brilliant Blue R- 250 staining solution Bio- Rad 1610436 For polyacrylamide gels Other Amersham Protran Premium 0.45 nitrocellulose membrane Cytiva 10600047 For Western blots Other Glutathione Sepharose 4B Cytiva 17075601 Used for purification of condensin I Other HiTrap Q HP 1 mL Cytiva 17115301 Used for purification of condensins Other StrepTactin Sepharose HP Cytiva 28- 9355- 99 Used for purification of condensin II Other DAPI Roche 10236276001 (2 μg/mL) Other ATP Sigma- Aldrich A2383 Used in ATPase assay and Npm2- treated chromatin binding assay Other Dynabeads Protein A ThermoFisher 10002D For immunodepletion with Xenopus egg extract Other Alexa Flour 488- conjugated streptavidin ThermoFisher S32354 IF (1/2000) SMC: structural maintenance of chromosomes. ..

    Staining:

    Article Title: Molecular dissection of condensin II-mediated chromosome assembly using in vitro assays
    Article Snippet: Reagent type (species) or resource Designation Source or reference Identifiers Additional information Cell line (Spodoptera frugiperda) Sf9 insect cells ThermoFisher 11496015 Cell line (Spodoptera frugiperda) High five insect cells ThermoFisher B85502 Strain, strain background (Escherichia coli) Max efficiency DH10Bac competent cells ThermoFisher 10361012 Strain, strain background (Escherichia coli) BL21(DE3) competent cells with lab cultured cells N/A Electrocompetent cells Biological sample (Xenopus laevis) Xenopus laevis egg Hamamatsu SeibutsuKyozai RRID: NXR 0.031 Female, adult frogs Biological sample (Xenopus laevis) Xenopus laevis sperm nuclei Hamamatsu SeibutsuKyozai RRID: NXR 0.031 Male, adult frogs Biological sample (Mus musculus) Mus musculus sperm nuclei Shintomi et al., 2017; isolated from Mus musculus cauda epididymis (BALB/ c×C57BL/6 J)F1 N/A Male, adult mouse Antibody anti- Xenopus topoisomerase IIα (rabbit polyclonal serum) Hirano and Mitchison, 1994 in house: αC1- 6 WB (1/2000) Antibody anti- XCAP- D2 (rabbit polyclonal) Hirano et al., 1997 in house: AfR16L WB (1.0 μg/mL) Antibody anti- XCAP- G (rabbit polyclonal) Hirano et al., 1997 in house: AfR113L WB (1.0 μg/mL) Yoshida et al. eLife 2022;11:e78984. .. DOI: https://doi.org/10.7554/eLife.78984 19 of 27 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Peptide, recombinant protein EcoRI restriction enzyme TaKaRa 1040 Peptide, recombinant protein NotI restriction enzyme TaKaRa 1166 Peptide, recombinant protein SpeI restriction enzyme TaKaRa 1086 Peptide, recombinant protein T4 polynucleotide kinase TaKaRa 2021 Commercial assay or kit QuikChange II XL Site- Directed Mutagenesis Kit Agilent Technologies 200522 Commercial assay or kit DNA Ligation Kit TaKaRa 6022 Commercial assay or kit EnzChek Phosphate Assay Kit ThermoFisher E6646 Software, algorithm UNICORN 7 Cytiva N/A Software, algorithm Prism 8 GraphPad N/A Software, algorithm Excel Microsoft N/A Software, algorithm Olympus cellSens Dimensions Olympus N/A Software, algorithm Photoshop Adobe N/A Software, algorithm ImageJ Schneider et al., 2012 https://imagej.nih. gov/ij/ Software, algorithm SparkControl Tecan Life Sciences N/A Other Coomassie Brilliant Blue R- 250 staining solution Bio- Rad 1610436 For polyacrylamide gels Other Amersham Protran Premium 0.45 nitrocellulose membrane Cytiva 10600047 For Western blots Other Glutathione Sepharose 4B Cytiva 17075601 Used for purification of condensin I Other HiTrap Q HP 1 mL Cytiva 17115301 Used for purification of condensins Other StrepTactin Sepharose HP Cytiva 28- 9355- 99 Used for purification of condensin II Other DAPI Roche 10236276001 (2 μg/mL) Other ATP Sigma- Aldrich A2383 Used in ATPase assay and Npm2- treated chromatin binding assay Other Dynabeads Protein A ThermoFisher 10002D For immunodepletion with Xenopus egg extract Other Alexa Flour 488- conjugated streptavidin ThermoFisher S32354 IF (1/2000) SMC: structural maintenance of chromosomes. ..

    Membrane:

    Article Title: Molecular dissection of condensin II-mediated chromosome assembly using in vitro assays
    Article Snippet: Reagent type (species) or resource Designation Source or reference Identifiers Additional information Cell line (Spodoptera frugiperda) Sf9 insect cells ThermoFisher 11496015 Cell line (Spodoptera frugiperda) High five insect cells ThermoFisher B85502 Strain, strain background (Escherichia coli) Max efficiency DH10Bac competent cells ThermoFisher 10361012 Strain, strain background (Escherichia coli) BL21(DE3) competent cells with lab cultured cells N/A Electrocompetent cells Biological sample (Xenopus laevis) Xenopus laevis egg Hamamatsu SeibutsuKyozai RRID: NXR 0.031 Female, adult frogs Biological sample (Xenopus laevis) Xenopus laevis sperm nuclei Hamamatsu SeibutsuKyozai RRID: NXR 0.031 Male, adult frogs Biological sample (Mus musculus) Mus musculus sperm nuclei Shintomi et al., 2017; isolated from Mus musculus cauda epididymis (BALB/ c×C57BL/6 J)F1 N/A Male, adult mouse Antibody anti- Xenopus topoisomerase IIα (rabbit polyclonal serum) Hirano and Mitchison, 1994 in house: αC1- 6 WB (1/2000) Antibody anti- XCAP- D2 (rabbit polyclonal) Hirano et al., 1997 in house: AfR16L WB (1.0 μg/mL) Antibody anti- XCAP- G (rabbit polyclonal) Hirano et al., 1997 in house: AfR113L WB (1.0 μg/mL) Yoshida et al. eLife 2022;11:e78984. .. DOI: https://doi.org/10.7554/eLife.78984 19 of 27 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Peptide, recombinant protein EcoRI restriction enzyme TaKaRa 1040 Peptide, recombinant protein NotI restriction enzyme TaKaRa 1166 Peptide, recombinant protein SpeI restriction enzyme TaKaRa 1086 Peptide, recombinant protein T4 polynucleotide kinase TaKaRa 2021 Commercial assay or kit QuikChange II XL Site- Directed Mutagenesis Kit Agilent Technologies 200522 Commercial assay or kit DNA Ligation Kit TaKaRa 6022 Commercial assay or kit EnzChek Phosphate Assay Kit ThermoFisher E6646 Software, algorithm UNICORN 7 Cytiva N/A Software, algorithm Prism 8 GraphPad N/A Software, algorithm Excel Microsoft N/A Software, algorithm Olympus cellSens Dimensions Olympus N/A Software, algorithm Photoshop Adobe N/A Software, algorithm ImageJ Schneider et al., 2012 https://imagej.nih. gov/ij/ Software, algorithm SparkControl Tecan Life Sciences N/A Other Coomassie Brilliant Blue R- 250 staining solution Bio- Rad 1610436 For polyacrylamide gels Other Amersham Protran Premium 0.45 nitrocellulose membrane Cytiva 10600047 For Western blots Other Glutathione Sepharose 4B Cytiva 17075601 Used for purification of condensin I Other HiTrap Q HP 1 mL Cytiva 17115301 Used for purification of condensins Other StrepTactin Sepharose HP Cytiva 28- 9355- 99 Used for purification of condensin II Other DAPI Roche 10236276001 (2 μg/mL) Other ATP Sigma- Aldrich A2383 Used in ATPase assay and Npm2- treated chromatin binding assay Other Dynabeads Protein A ThermoFisher 10002D For immunodepletion with Xenopus egg extract Other Alexa Flour 488- conjugated streptavidin ThermoFisher S32354 IF (1/2000) SMC: structural maintenance of chromosomes. ..

    Western Blot:

    Article Title: Molecular dissection of condensin II-mediated chromosome assembly using in vitro assays
    Article Snippet: Reagent type (species) or resource Designation Source or reference Identifiers Additional information Cell line (Spodoptera frugiperda) Sf9 insect cells ThermoFisher 11496015 Cell line (Spodoptera frugiperda) High five insect cells ThermoFisher B85502 Strain, strain background (Escherichia coli) Max efficiency DH10Bac competent cells ThermoFisher 10361012 Strain, strain background (Escherichia coli) BL21(DE3) competent cells with lab cultured cells N/A Electrocompetent cells Biological sample (Xenopus laevis) Xenopus laevis egg Hamamatsu SeibutsuKyozai RRID: NXR 0.031 Female, adult frogs Biological sample (Xenopus laevis) Xenopus laevis sperm nuclei Hamamatsu SeibutsuKyozai RRID: NXR 0.031 Male, adult frogs Biological sample (Mus musculus) Mus musculus sperm nuclei Shintomi et al., 2017; isolated from Mus musculus cauda epididymis (BALB/ c×C57BL/6 J)F1 N/A Male, adult mouse Antibody anti- Xenopus topoisomerase IIα (rabbit polyclonal serum) Hirano and Mitchison, 1994 in house: αC1- 6 WB (1/2000) Antibody anti- XCAP- D2 (rabbit polyclonal) Hirano et al., 1997 in house: AfR16L WB (1.0 μg/mL) Antibody anti- XCAP- G (rabbit polyclonal) Hirano et al., 1997 in house: AfR113L WB (1.0 μg/mL) Yoshida et al. eLife 2022;11:e78984. .. DOI: https://doi.org/10.7554/eLife.78984 19 of 27 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Peptide, recombinant protein EcoRI restriction enzyme TaKaRa 1040 Peptide, recombinant protein NotI restriction enzyme TaKaRa 1166 Peptide, recombinant protein SpeI restriction enzyme TaKaRa 1086 Peptide, recombinant protein T4 polynucleotide kinase TaKaRa 2021 Commercial assay or kit QuikChange II XL Site- Directed Mutagenesis Kit Agilent Technologies 200522 Commercial assay or kit DNA Ligation Kit TaKaRa 6022 Commercial assay or kit EnzChek Phosphate Assay Kit ThermoFisher E6646 Software, algorithm UNICORN 7 Cytiva N/A Software, algorithm Prism 8 GraphPad N/A Software, algorithm Excel Microsoft N/A Software, algorithm Olympus cellSens Dimensions Olympus N/A Software, algorithm Photoshop Adobe N/A Software, algorithm ImageJ Schneider et al., 2012 https://imagej.nih. gov/ij/ Software, algorithm SparkControl Tecan Life Sciences N/A Other Coomassie Brilliant Blue R- 250 staining solution Bio- Rad 1610436 For polyacrylamide gels Other Amersham Protran Premium 0.45 nitrocellulose membrane Cytiva 10600047 For Western blots Other Glutathione Sepharose 4B Cytiva 17075601 Used for purification of condensin I Other HiTrap Q HP 1 mL Cytiva 17115301 Used for purification of condensins Other StrepTactin Sepharose HP Cytiva 28- 9355- 99 Used for purification of condensin II Other DAPI Roche 10236276001 (2 μg/mL) Other ATP Sigma- Aldrich A2383 Used in ATPase assay and Npm2- treated chromatin binding assay Other Dynabeads Protein A ThermoFisher 10002D For immunodepletion with Xenopus egg extract Other Alexa Flour 488- conjugated streptavidin ThermoFisher S32354 IF (1/2000) SMC: structural maintenance of chromosomes. ..

    Purification:

    Article Title: Molecular dissection of condensin II-mediated chromosome assembly using in vitro assays
    Article Snippet: Reagent type (species) or resource Designation Source or reference Identifiers Additional information Cell line (Spodoptera frugiperda) Sf9 insect cells ThermoFisher 11496015 Cell line (Spodoptera frugiperda) High five insect cells ThermoFisher B85502 Strain, strain background (Escherichia coli) Max efficiency DH10Bac competent cells ThermoFisher 10361012 Strain, strain background (Escherichia coli) BL21(DE3) competent cells with lab cultured cells N/A Electrocompetent cells Biological sample (Xenopus laevis) Xenopus laevis egg Hamamatsu SeibutsuKyozai RRID: NXR 0.031 Female, adult frogs Biological sample (Xenopus laevis) Xenopus laevis sperm nuclei Hamamatsu SeibutsuKyozai RRID: NXR 0.031 Male, adult frogs Biological sample (Mus musculus) Mus musculus sperm nuclei Shintomi et al., 2017; isolated from Mus musculus cauda epididymis (BALB/ c×C57BL/6 J)F1 N/A Male, adult mouse Antibody anti- Xenopus topoisomerase IIα (rabbit polyclonal serum) Hirano and Mitchison, 1994 in house: αC1- 6 WB (1/2000) Antibody anti- XCAP- D2 (rabbit polyclonal) Hirano et al., 1997 in house: AfR16L WB (1.0 μg/mL) Antibody anti- XCAP- G (rabbit polyclonal) Hirano et al., 1997 in house: AfR113L WB (1.0 μg/mL) Yoshida et al. eLife 2022;11:e78984. .. DOI: https://doi.org/10.7554/eLife.78984 19 of 27 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Peptide, recombinant protein EcoRI restriction enzyme TaKaRa 1040 Peptide, recombinant protein NotI restriction enzyme TaKaRa 1166 Peptide, recombinant protein SpeI restriction enzyme TaKaRa 1086 Peptide, recombinant protein T4 polynucleotide kinase TaKaRa 2021 Commercial assay or kit QuikChange II XL Site- Directed Mutagenesis Kit Agilent Technologies 200522 Commercial assay or kit DNA Ligation Kit TaKaRa 6022 Commercial assay or kit EnzChek Phosphate Assay Kit ThermoFisher E6646 Software, algorithm UNICORN 7 Cytiva N/A Software, algorithm Prism 8 GraphPad N/A Software, algorithm Excel Microsoft N/A Software, algorithm Olympus cellSens Dimensions Olympus N/A Software, algorithm Photoshop Adobe N/A Software, algorithm ImageJ Schneider et al., 2012 https://imagej.nih. gov/ij/ Software, algorithm SparkControl Tecan Life Sciences N/A Other Coomassie Brilliant Blue R- 250 staining solution Bio- Rad 1610436 For polyacrylamide gels Other Amersham Protran Premium 0.45 nitrocellulose membrane Cytiva 10600047 For Western blots Other Glutathione Sepharose 4B Cytiva 17075601 Used for purification of condensin I Other HiTrap Q HP 1 mL Cytiva 17115301 Used for purification of condensins Other StrepTactin Sepharose HP Cytiva 28- 9355- 99 Used for purification of condensin II Other DAPI Roche 10236276001 (2 μg/mL) Other ATP Sigma- Aldrich A2383 Used in ATPase assay and Npm2- treated chromatin binding assay Other Dynabeads Protein A ThermoFisher 10002D For immunodepletion with Xenopus egg extract Other Alexa Flour 488- conjugated streptavidin ThermoFisher S32354 IF (1/2000) SMC: structural maintenance of chromosomes. ..

    ATPase Assay:

    Article Title: Molecular dissection of condensin II-mediated chromosome assembly using in vitro assays
    Article Snippet: Reagent type (species) or resource Designation Source or reference Identifiers Additional information Cell line (Spodoptera frugiperda) Sf9 insect cells ThermoFisher 11496015 Cell line (Spodoptera frugiperda) High five insect cells ThermoFisher B85502 Strain, strain background (Escherichia coli) Max efficiency DH10Bac competent cells ThermoFisher 10361012 Strain, strain background (Escherichia coli) BL21(DE3) competent cells with lab cultured cells N/A Electrocompetent cells Biological sample (Xenopus laevis) Xenopus laevis egg Hamamatsu SeibutsuKyozai RRID: NXR 0.031 Female, adult frogs Biological sample (Xenopus laevis) Xenopus laevis sperm nuclei Hamamatsu SeibutsuKyozai RRID: NXR 0.031 Male, adult frogs Biological sample (Mus musculus) Mus musculus sperm nuclei Shintomi et al., 2017; isolated from Mus musculus cauda epididymis (BALB/ c×C57BL/6 J)F1 N/A Male, adult mouse Antibody anti- Xenopus topoisomerase IIα (rabbit polyclonal serum) Hirano and Mitchison, 1994 in house: αC1- 6 WB (1/2000) Antibody anti- XCAP- D2 (rabbit polyclonal) Hirano et al., 1997 in house: AfR16L WB (1.0 μg/mL) Antibody anti- XCAP- G (rabbit polyclonal) Hirano et al., 1997 in house: AfR113L WB (1.0 μg/mL) Yoshida et al. eLife 2022;11:e78984. .. DOI: https://doi.org/10.7554/eLife.78984 19 of 27 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Peptide, recombinant protein EcoRI restriction enzyme TaKaRa 1040 Peptide, recombinant protein NotI restriction enzyme TaKaRa 1166 Peptide, recombinant protein SpeI restriction enzyme TaKaRa 1086 Peptide, recombinant protein T4 polynucleotide kinase TaKaRa 2021 Commercial assay or kit QuikChange II XL Site- Directed Mutagenesis Kit Agilent Technologies 200522 Commercial assay or kit DNA Ligation Kit TaKaRa 6022 Commercial assay or kit EnzChek Phosphate Assay Kit ThermoFisher E6646 Software, algorithm UNICORN 7 Cytiva N/A Software, algorithm Prism 8 GraphPad N/A Software, algorithm Excel Microsoft N/A Software, algorithm Olympus cellSens Dimensions Olympus N/A Software, algorithm Photoshop Adobe N/A Software, algorithm ImageJ Schneider et al., 2012 https://imagej.nih. gov/ij/ Software, algorithm SparkControl Tecan Life Sciences N/A Other Coomassie Brilliant Blue R- 250 staining solution Bio- Rad 1610436 For polyacrylamide gels Other Amersham Protran Premium 0.45 nitrocellulose membrane Cytiva 10600047 For Western blots Other Glutathione Sepharose 4B Cytiva 17075601 Used for purification of condensin I Other HiTrap Q HP 1 mL Cytiva 17115301 Used for purification of condensins Other StrepTactin Sepharose HP Cytiva 28- 9355- 99 Used for purification of condensin II Other DAPI Roche 10236276001 (2 μg/mL) Other ATP Sigma- Aldrich A2383 Used in ATPase assay and Npm2- treated chromatin binding assay Other Dynabeads Protein A ThermoFisher 10002D For immunodepletion with Xenopus egg extract Other Alexa Flour 488- conjugated streptavidin ThermoFisher S32354 IF (1/2000) SMC: structural maintenance of chromosomes. ..

    Binding Assay:

    Article Title: Molecular dissection of condensin II-mediated chromosome assembly using in vitro assays
    Article Snippet: Reagent type (species) or resource Designation Source or reference Identifiers Additional information Cell line (Spodoptera frugiperda) Sf9 insect cells ThermoFisher 11496015 Cell line (Spodoptera frugiperda) High five insect cells ThermoFisher B85502 Strain, strain background (Escherichia coli) Max efficiency DH10Bac competent cells ThermoFisher 10361012 Strain, strain background (Escherichia coli) BL21(DE3) competent cells with lab cultured cells N/A Electrocompetent cells Biological sample (Xenopus laevis) Xenopus laevis egg Hamamatsu SeibutsuKyozai RRID: NXR 0.031 Female, adult frogs Biological sample (Xenopus laevis) Xenopus laevis sperm nuclei Hamamatsu SeibutsuKyozai RRID: NXR 0.031 Male, adult frogs Biological sample (Mus musculus) Mus musculus sperm nuclei Shintomi et al., 2017; isolated from Mus musculus cauda epididymis (BALB/ c×C57BL/6 J)F1 N/A Male, adult mouse Antibody anti- Xenopus topoisomerase IIα (rabbit polyclonal serum) Hirano and Mitchison, 1994 in house: αC1- 6 WB (1/2000) Antibody anti- XCAP- D2 (rabbit polyclonal) Hirano et al., 1997 in house: AfR16L WB (1.0 μg/mL) Antibody anti- XCAP- G (rabbit polyclonal) Hirano et al., 1997 in house: AfR113L WB (1.0 μg/mL) Yoshida et al. eLife 2022;11:e78984. .. DOI: https://doi.org/10.7554/eLife.78984 19 of 27 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Peptide, recombinant protein EcoRI restriction enzyme TaKaRa 1040 Peptide, recombinant protein NotI restriction enzyme TaKaRa 1166 Peptide, recombinant protein SpeI restriction enzyme TaKaRa 1086 Peptide, recombinant protein T4 polynucleotide kinase TaKaRa 2021 Commercial assay or kit QuikChange II XL Site- Directed Mutagenesis Kit Agilent Technologies 200522 Commercial assay or kit DNA Ligation Kit TaKaRa 6022 Commercial assay or kit EnzChek Phosphate Assay Kit ThermoFisher E6646 Software, algorithm UNICORN 7 Cytiva N/A Software, algorithm Prism 8 GraphPad N/A Software, algorithm Excel Microsoft N/A Software, algorithm Olympus cellSens Dimensions Olympus N/A Software, algorithm Photoshop Adobe N/A Software, algorithm ImageJ Schneider et al., 2012 https://imagej.nih. gov/ij/ Software, algorithm SparkControl Tecan Life Sciences N/A Other Coomassie Brilliant Blue R- 250 staining solution Bio- Rad 1610436 For polyacrylamide gels Other Amersham Protran Premium 0.45 nitrocellulose membrane Cytiva 10600047 For Western blots Other Glutathione Sepharose 4B Cytiva 17075601 Used for purification of condensin I Other HiTrap Q HP 1 mL Cytiva 17115301 Used for purification of condensins Other StrepTactin Sepharose HP Cytiva 28- 9355- 99 Used for purification of condensin II Other DAPI Roche 10236276001 (2 μg/mL) Other ATP Sigma- Aldrich A2383 Used in ATPase assay and Npm2- treated chromatin binding assay Other Dynabeads Protein A ThermoFisher 10002D For immunodepletion with Xenopus egg extract Other Alexa Flour 488- conjugated streptavidin ThermoFisher S32354 IF (1/2000) SMC: structural maintenance of chromosomes. ..

    Immunodepletion:

    Article Title: Molecular dissection of condensin II-mediated chromosome assembly using in vitro assays
    Article Snippet: Reagent type (species) or resource Designation Source or reference Identifiers Additional information Cell line (Spodoptera frugiperda) Sf9 insect cells ThermoFisher 11496015 Cell line (Spodoptera frugiperda) High five insect cells ThermoFisher B85502 Strain, strain background (Escherichia coli) Max efficiency DH10Bac competent cells ThermoFisher 10361012 Strain, strain background (Escherichia coli) BL21(DE3) competent cells with lab cultured cells N/A Electrocompetent cells Biological sample (Xenopus laevis) Xenopus laevis egg Hamamatsu SeibutsuKyozai RRID: NXR 0.031 Female, adult frogs Biological sample (Xenopus laevis) Xenopus laevis sperm nuclei Hamamatsu SeibutsuKyozai RRID: NXR 0.031 Male, adult frogs Biological sample (Mus musculus) Mus musculus sperm nuclei Shintomi et al., 2017; isolated from Mus musculus cauda epididymis (BALB/ c×C57BL/6 J)F1 N/A Male, adult mouse Antibody anti- Xenopus topoisomerase IIα (rabbit polyclonal serum) Hirano and Mitchison, 1994 in house: αC1- 6 WB (1/2000) Antibody anti- XCAP- D2 (rabbit polyclonal) Hirano et al., 1997 in house: AfR16L WB (1.0 μg/mL) Antibody anti- XCAP- G (rabbit polyclonal) Hirano et al., 1997 in house: AfR113L WB (1.0 μg/mL) Yoshida et al. eLife 2022;11:e78984. .. DOI: https://doi.org/10.7554/eLife.78984 19 of 27 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Peptide, recombinant protein EcoRI restriction enzyme TaKaRa 1040 Peptide, recombinant protein NotI restriction enzyme TaKaRa 1166 Peptide, recombinant protein SpeI restriction enzyme TaKaRa 1086 Peptide, recombinant protein T4 polynucleotide kinase TaKaRa 2021 Commercial assay or kit QuikChange II XL Site- Directed Mutagenesis Kit Agilent Technologies 200522 Commercial assay or kit DNA Ligation Kit TaKaRa 6022 Commercial assay or kit EnzChek Phosphate Assay Kit ThermoFisher E6646 Software, algorithm UNICORN 7 Cytiva N/A Software, algorithm Prism 8 GraphPad N/A Software, algorithm Excel Microsoft N/A Software, algorithm Olympus cellSens Dimensions Olympus N/A Software, algorithm Photoshop Adobe N/A Software, algorithm ImageJ Schneider et al., 2012 https://imagej.nih. gov/ij/ Software, algorithm SparkControl Tecan Life Sciences N/A Other Coomassie Brilliant Blue R- 250 staining solution Bio- Rad 1610436 For polyacrylamide gels Other Amersham Protran Premium 0.45 nitrocellulose membrane Cytiva 10600047 For Western blots Other Glutathione Sepharose 4B Cytiva 17075601 Used for purification of condensin I Other HiTrap Q HP 1 mL Cytiva 17115301 Used for purification of condensins Other StrepTactin Sepharose HP Cytiva 28- 9355- 99 Used for purification of condensin II Other DAPI Roche 10236276001 (2 μg/mL) Other ATP Sigma- Aldrich A2383 Used in ATPase assay and Npm2- treated chromatin binding assay Other Dynabeads Protein A ThermoFisher 10002D For immunodepletion with Xenopus egg extract Other Alexa Flour 488- conjugated streptavidin ThermoFisher S32354 IF (1/2000) SMC: structural maintenance of chromosomes. ..



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